| 范佳,王军威,孙君志,等.有氧运动和抗阻运动干预对结肠癌CT26荷瘤小鼠骨骼肌肌力及心脏功能的影响[J].中华物理医学与康复杂志,2026,48(9):769-777 |
| 有氧运动和抗阻运动干预对结肠癌CT26荷瘤小鼠骨骼肌肌力及心脏功能的影响 |
|
| |
| DOI：10.3760/cma.j.cn421666-20251125-01045 |
| 中文关键词: 有氧运动 抗阻运动 肌力 心脏功能 |
| 英文关键词: Aerobic exercise Resistance movement Muscle strength Cardiac function |
| 基金项目:四川省自然科学基金（2026NSFC0497）；四川省科技厅课题（2022JDRC0137） |
|
| 摘要点击次数: 181 |
| 全文下载次数: 150 |
| 中文摘要: |
| 目的 观察有氧运动与抗阻运动干预对结肠癌CT26荷瘤小鼠骨骼肌肌力及心脏功能的影响。 方法 将24只雄性BALB/c小鼠适应性喂养7 d后，按照随机数字表法将其分为对照组（NC组）、模型组（M组）、有氧运动+模型组（AM组）和抗阻运动+模型组（RM组），每组6只。所有组均进行4周运动预处理，第4周末对M组、AM组和RM组皮下接种CT26细胞株建立结肠癌荷瘤模型，NC组同法注射生理盐水。第5～8周，AM组采用跑台训练进行有氧运动，RM组采用负重爬梯训练进行抗阻运动。第1～8周，每周检测小鼠抓力，运动干预结束后利用转棒疲劳仪测试耐力。末次训练结束24 h后，采用Vivid 7 dimension M型超声心动仪进行心功能检测，并取心脏组织行苏木精-伊红（HE）染色观察心肌病理形态变化。 结果 M组小鼠出现精神萎靡、动作迟缓、食欲下降、被毛无光泽、竖毛及形体瘦弱等异常情况。骨骼肌抓力结果显示：第1～4周，各组小鼠的抓力平稳增加；自第5周末起，4组小鼠的抓力出现显著差异，其中NC组与M组比较，差异有统计学意义（P<0.05）；M组与AM组、RM组比较，差异有统计学意义（P<0.05）；AM组与RM组比较，差异亦有统计学意义（P<0.05）；第6周末和第7周末，NC组与M组的抓力差异、M组与AM组及RM组的抓力差异均有统计学意义（P<0.05）；第8周末，仅NC组与M组的抓力差异有统计学意义（P<0.05）。心脏病理形态结果显示：与NC组相比，M组偶见心肌纤维明显变细、断裂及排列紊乱，AM组和RM组仅偶见心肌纤维变细。超声心动图检测结果显示：NC组与M组的每搏输出量（SV）、左室舒张末后壁厚度（LVPWd）、左室舒张末内径（LVIDd）和左室舒张末期容积（EDV）比较，差异有统计学意义（P<0.05）；M组与AM组、RM组的SV比较，差异有统计学意义（P<0.05）。 结论 有氧运动和抗阻运动均能延缓结肠癌CT26荷瘤小鼠骨骼肌肌力的下降，并改善其心脏功能。 |
| 英文摘要: |
| Objective To observe the effects of aerobic exercise and resistance exercise interventions on skeletal muscle strength and cardiac function in colon cancer CT26 tumor-bearing mice. Methods Twenty-four male BALB/c mice were randomly assigned to four groups (n=6 per group) after a 7-day acclimatization period: control (NC) group, tumor-bearing model (M) group, aerobic exercise plus model (AM) group, and resistance exercise plus model (RM) group. All groups underwent a 4-week pre-conditioning exercise protocol. At the end of week 4, mice in the M, AM, and RM groups were subcutaneously inoculated with CT26 colon cancer cells to establish the tumor-bearing model, while the NC group received an equivalent volume of saline. From weeks 5 to 8, the AM group performed treadmill-based aerobic exercise, and the RM group underwent weight-loaded ladder climbing for resistance training. Grip strength was measured weekly from week 1 to week 8. Endurance was assessed using a rotarod fatigue test following the intervention. Twenty-four hours after the final training session, cardiac function was evaluated via echocardiography using a Vivid 7 Dimension M-mode system. Moreover, myocardial tissue was harvested and stained with hematoxylin and eosin for histopathological examination. Results Mice in the M group exhibited typical cancer cachexia symptoms, including lethargy, delayed movement, reduced appetite, dull and erect fur, and significant weight loss. Grip strength increased steadily in all groups during the first 4 weeks. From week 5 onward, significant intergroup differences emerged: NC vs M, M vs AM/RM, and AM vs RM all showed statistically significant differences (P<0.05). These differences persisted at weeks 6 and 7. By week 8, only the NC vs M comparison remained statistically significant. Histopathological analysis revealed that compared to the NC group, the M group exhibited myocardial fiber thinning, fragmentation, and disarray; these changes were markedly attenuated in the AM and RM groups. Echocardiographic parameters, including stroke volume (SV), left ventricular posterior wall thickness at end-diastole (LVPWd), left ventricular internal diameter at end-diastole (LVIDd), and end-diastolic volume (EDV), were significantly impaired in the M group versus NC (P<0.05). Both AM and RM groups demonstrated significant improvement in SV compared to the M group (P<0.05). Conclusions Both aerobic and resistance exercise interventions effectively attenuate the decline in skeletal muscle strength and improve cardiac function in CT26 colon cancer-bearing mice. |
|
查看全文
下载PDF阅读器 HTML全文 |
| 关闭 |
|
|
|